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Master's Dissertation
DOI
https://doi.org/10.11606/D.11.1973.tde-20240301-152130
Document
Author
Full name
Antonio de Oliveira Lobão
Institute/School/College
Date of Defense
Published
Piracicaba, 1973
Supervisor
Title in Portuguese
Distribuição de fósforo radioativo (32P) em tecidos de ovinos
Keywords in Portuguese
FÓSFORO-32
OVINOS
RADIOISÓTOPOS
TECIDOS ANIMAIS
Abstract in Portuguese
Doze carneiros, machos da raça Corriedale foram separados em dois grupos conforme a idade média (114 dias e 208 dias). Os animais de cada grupo foram sorteados dois a dois conforme o peso e constituíram as repetições. Após um período de adaptação de 7 dias, ocasião em que continuavam recebendo suplementação mineral à vontade, eram submetidos a uma injeção endovenosa de 1 ml de Na2H32PO4. No volume aplicado a radioatividade variou com o decorrer do tempo: de 500 µci a 225,9 µci para os 6 animais da primeira fase, e de 500 µci à 87,5 µci para os 6 animais da segunda fase. Após 8, 16 e 24 horas da aplicação da injeção, os animais foram anestesiados e sacrificados por sangria total. Além do soro e sangue, foram coletados fragmentos de língua, cérebro, coração, diafragma, baço, rim, fígado e osso. Os tecidos foram secos, incinerados, e os extratos obtidos com ácido sulfúrico levados ao cintilador líquido e sua radioatividade detectada através da radiação Cerenkov. O teor sérico de fósforo inorgânico dos animais foi determinado com material obtido na véspera do experimento. Os animais mais novos apresentaram menor teor sérico de fósforo inorgânico e maior retenção de 32P em seus tecidos. A retenção do 32P nos tecidos decresceu na seguinte ordem: fígado, rim, baço, coração e língua. O cérebro sempre apresentou menor retenção. Essa ordem não foi influenciada pela idade dos animais, pelo tempo experimental e nem pelo nível sérico de fósforo inorgânico. O montante do fósforo retido pelos tecidos foi influenciado não só pela idade dos animais mas também pelo teor de fósforo no sangue e para alguns tecidos, também pelo tempo experimental.
Abstract in English
Twelve male lambs (Corriedale) were separated in two groups according to their average age (114 days and 208 days). The animals of each group were selected in twos according to weight to provide duplicates. After a 7 day period of adaptation, during which time the lambs received an unrestricted mineral supplement, they were given intravenous injections of 1 ml of Na2H32PO4. The amount of radioactivity administered varied with time; from 500 µci to 225,9 µci for the 6 younger animals, and from 500 µci to 87.5 µci for the 6 older ones. At 8, 16, and 24 hours after the injections, the animals were anesthetized and sacrificed by bleeding. As well as the serum and blood, pieces, of tongue, brain, heart, diaphragm spleen, kidney, liver and bone were collected. There tissues were dried, incinerated and the extracts obtained with H2SO4 counted by Cerenkov radiation in a liquid scintillation counter. The level of inorganic phosphorus in the serum was determined with material obtained the day before the experiment. The younger animals had lower levels of serum inorganic phosphorus and retained higher levels of 32P in their tissues. The isotope was found in a decreasing order in the following tissues: liver, kidney, spleen, heart and tongue. The brain presented the least retention. The order of magnitude by which the tissues retained the isotope was not influenced by the age of the animals, by the experimental period, or by the level of serum inorganic phosphorus. The amount of phosphorus retained by the tissues was affected not only by the age of the animals, but also by the level of phosphorus in the blood and, for some tissues, by the experimental period.
 
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Publishing Date
2024-03-14
 
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