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Master's Dissertation
DOI
https://doi.org/10.11606/D.11.1973.tde-20240301-154002
Document
Author
Full name
Peri Veiga
Institute/School/College
Date of Defense
Published
Piracicaba, 1973
Supervisor
Title in Portuguese
Cercospora sojina Hara: obtenção de inóculo, inoculação e avaliação da resistência em soja (Glycine max (L) Merr.)
Keywords in Portuguese
FUNGOS FITOPATOGÊNICOS
INOCULAÇÃO
MANCHA OLHO-DE-RÃ
RESISTÊNCIA GENÉTICA VEGETAL
SOJA
Abstract in Portuguese
Dois isolados de Cercospora sojina Hara foram cultivados em diferentes condições de meio de cultura e regime luminoso chegando-se à conclusão que a esporulação é melhor em: a) V - 8A (Suco V - 8 agar) e FAA (Farinha de aveia agar) do que em BDA (Batata dextrose agar) e FCA (Folhas de cenoura agar); b) em luz (fluorescente General Electric de 40 Watts) alternada do que em luz e escuro contínuos; c) no isolado 1 do que no isolado 2. Inoculando-se os dois isolados, cada um de persi, em três variedades de soja (Bragg, Davis e Halle - 7), em três concentrações de inóculo (10.000, 20.000 e 40.000 esporos por ml) e usando-se três métodos de avaliação (número de lesões, tamanho de lesões e superfície foliar afetada), chegou-se as seguintes conclusões: 1) por qualquer método de avaliação, os fatores: isolado, variedade e concentração são importantes; 2) o método de avaliação baseado em número de lesões pode levar a resultados diferentes daquele baseado em tamanho, pelo que se propõe um índice baseado em área foliar atacada; 3) qualquer que seja o método de avaliação a variedade Davis é a mais resistente.
Abstract in English
Two isolates of Cercospora sojina Hara were cultivated under different conditions of substrate and light. It was concluded that sporulation (conidia) was better in V - 8 agar and oatmeal agar than in potato dextrose agar and carrot agar (extract of leaves). Sporulation in light (Fluorescent G. E. 40 watts) and dark alternating was better than continuous light or continuous darkness. Isolate 1, in general, sporulated better than isolate 2. Three varieties of soybean (Bragg, Davis and Halle - 7), were inoculated with each of the two isolates at concentrations of 10,000, 20,000 and 40,000 conidia per ml. Three methods of evaluation of symptom development were used (number of lesions, size of lesions and amount of symptoms of each leaf affected). From the results the following conclusions are drawn: 1. whatever method of evaluation was used - the isolate, the variety and the conidial concentration are important; 2. the evaluation based on number of lesions can give results different than those based on size of lesions. Because of this an index is suggested (used in this research) based on the area of leaf affected; 3. whatever the method of evaluation, the variety Davis is considered the most resistant under the conditions of the experiments.
 
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Publishing Date
2024-03-14
 
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